The University of Chicago Flow Cytometry Core facility is a shared research facility dedicated to the education and further advancement of flow cytometry technologies used in research and clinical studies at the University of Chicago. The facility staff is on hand to maintain the instrumentation, provide services of training, general education, and consultation.
Since the advent of hybridoma technology, introduced by Kohler and Milstein in 1976, monoclonal antibodies have served as complementary elements in the areas of genetic research, immunology, and therapeutic applications. At the University of Chicago, this technology was pioneered by Dr. Frank Fitch in the Department of Pathology, who generated many monoclonal antibodies still used today in the field of Immunology. In 1995, the Frank W. Fitch Monoclonal Antibody Facility was created to promote this technology and make core services available to the University of Chicago community. The facility's goal is to work closely with investigators to offer comprehensive hybridoma and protein production services, using state-of-the-art technology, suited to the specific basic science research or clinical needs of the investigator.
The Frank W. Fitch Monoclonal Antibody Facility continues to provide a growing range of standard and custom services related to monoclonal antibodies, protein purification and conjugation, tissue culture technique and frozen cell storage. We can offer advice on the development of custom murine ELISA assays and we have extensive experience in the area of hamster ELISAs. Our collection of hybridomas and their related antibodies is continuing to expand and the cost per milligram is only a fraction of that charged by commercial sources.Antibody and protein production services include:
- Antibody/Hybridoma Bank
- Protein Purification and Quantitation
- Antibody conjugation to Biotin and FITC
- Human or Murine Cytokine/Lymphokine assays for detection and analysis on BioPlex Reader
- Custom ELISA Assays
- Protocol Development or Established
- Teaching Novel Monoclonal Antibody Production Techniques and Strategies
- Custom Services
- Including prepartions of human or murine blood samples for Flow Cytometry Analysis
- Any questions on any service listed here or additional custom services please email Carol McShan
Many services can be provided to non-academic or Bio-Tech entities.
Acknowledgement of core facility contributions in publications is appreciated.
Cancer Center members should cite the Cancer Center Support Grant (P30CA014599) when publishing any work assisted by this core facility.