The Bio-Plex system (Bio-Rad), housed in the Flow Cytometry Core Facility, is a flexible, easy-to-use bead-based multiplex analysis system that permits the simultaneous analysis of up to 100 different biomolecules (proteins, peptides, or nucleic acids) in a single microtiter plate well. The most commonly used applications are cytokine detection and protein analysis for cell signaling investigations.

• Technology
• Driven by Luminex Xmap Technology
• Red laser differentiates bead subsets
• Green laser excites PE reporter
• Each bead can be functionalized using a different capture molecule, i.e. antibody, oligonucleotide, etc., depending on the assay chosen
• Performance
• Designed to run in 96-well format
• Uses as little as 50uL of sample volume
• Full plate screens in as little as 30minutes
• Applications
• Detect up to 26 Cytokines simultaneously from 50uL of supernatent, serum or lysates
• Dynamic ranges of 1pg/mL to 10,000pg/mL
• Detect signalling proteins from cell lysates in a multiplex fashion
• Allows SNP Analysis, pathogen detection, haplotyping and gene expression as well