High-Speed Cell Sorters have the capability to not just analyze the spectral and light scattering characteristics of cells, but also to physically fractionate the heterogeneous cell sample into as many as 4 highly purified populations, one cell at a time. The facility has, at this time, two such instruments. The sorters, though very similar in capabilities, have some specific differences that should be taken into consideration. Some things to consider include:
- Laser lines available: Dictates which fluorochromes can be used
- Optical filter layout: Determine which fluorochromes can be optimally detected
- Number of fluorescence detectors per laser: Specifies the maximum number of fluorescence parameters that can be simultaneously measured
- Throughput Rate and Sort Rate: This is generally dicatated by the concentration of the sample. How fast can you run your samples before you start aborting too many cells, and how to maximize yield by adjust the throughput rate.
- Parameter Sensitivity: Expressed per fluorescence channel as the channel Background (B) and Quantum Efficiency (Q)
- Nozzle sizes available: Cells of differing sizes require larger or smaller nozzle sizes respectively.
Please take a moment to learn about the characteristics of the instruments available from the facility. If you have questions about what instrument may be best for your application, please contact us.