Flow Cytometry Home
Antibody Titrations
After the post below regarding the Alexa Dye conjugations, I had a few questions from users, not about the Alexa Dyes available, but about the titration, and choosing the optimal antibody concentration. Titrating your antibodies will go a long way towards achieving good quality flow cytometry data. A properly titered antibody (click on title for more...)
Submitted: 24 June 2009, 9:51 am
Alexa Dye Antibody Conjugation
The Fitch Monoclonal Antibody Facility (MAF) on campus is now offering antibodies coupled to many of the Alexa dye flavors from Invitrogen. As you may know, the Alexa Dyes are small molecular weight fluorochromes capable of producing bright fluorescence and minimal photo-bleaching. These dyes are available in many wavelengths, and are named by their optimal excitation wavelength, for example Alexa 488 is optimally excited by the 488 laser, w (click on title for more...)
Submitted: 12 June 2009, 12:07 pm
Live from Beckman Coulter Big Bang Event
I'm here at the Embassy Suites in Rosemont, IL for the big event. I'll post some updates as the day progresses, and possibly a few pics as well. There's a pretty decent turnout, maybe about 50-75 people. We're just getting underway! More later.
0913: Picomotor driven laser steering optics for fine tuning laser alignment...awesome
0914: Gallios = Research Instrument, Navios = Clinical Instrument.
0917: A vendor has talked about instrument "sensitivity" without quoting MESF detection threshold values...There really is a God! It's good to see my ranting and raving has not been in vain.
0924: The boulevard. Coulter's answer to the "Octagon". 18 degree reflection system which minimizes light loss.
0930: Definitely some nice innovation on the light scattering front. "Enhanced FSC" mode allowed resolution of 0.4um beads from noise as well as from 0.5um beads.
0948: FCS file is packed with all the necessary informa (click on title for more...)
Submitted: 21 May 2009, 8:53 am
Never orphan your data again.
Picture this, you just finished running your samples on the Canto in R409, you've exported your data to the BDExport folder, and you reach into your pocket. Dang! You forgot your USB drive. You could walk all the way back to your lab in Billings in the pouring rain, but wait, you can just email the files to yourself. So, you log into the University's webmail system compose your email to yourself, attach your zipped folder of FCS files and you click send only to get a error that the file is too big to be sent over the University's network. What to do now? Do you just chance it and hope the computer doesn't crash before you come back tomorrow with your USB drive? I know I wouldn't. There is one other thing you can do. You can use NSIT's web-based file storage and sharing service. That's right, just by being a member of the University with a CNET ID, you can put up to 50MB 1GB of data on a server share and then pull it down at your desk. Just visit nsit.uchicag (click on title for more...)
Submitted: 15 May 2009, 5:57 pm
Annual Core Fair, Thursday 5/14/09, Free Food!
Well, it's that time of year again. The Office of Shared Research Facilities (OSRF) is hosting its annual Core Fair this Thursday from 12:00 to 1:30 PM in the Gordon Center Atrium. Technical Directors and staff from over 21 core facilities will be available to discuss services, hand out literature and display posters featuring Core capabilities. The Flow Facility will have a table set up to showcase some of its new toys and services including information on its newly upgraded FACSAria II, its 4-laser LSRII, the new 561nm laser line on the MoFlo, and perennial favorite, the BioPlex. So you'll definitely want to stop by and get any and all your questions answered on how the techno-savvy core facilities can assist you in your research. As always, if you have questions regarding the services available from any core facility, visit the OSRF web site at osrf.uchicago.edu, and for any questions regarding Flow Cytometry Instrumetation and Services, y (click on title for more...)
Submitted: 12 May 2009, 12:35 pm
The Flow Cytometry Facility at the University of Chicago provides instrumentation, education, and expertise for the University's Faculty. The mission of the facility is three-fold:
- Equip the facility with cutting-edge technology and expertise
- Pursue technology and application development
- Provide instrument and applcation training and education
We encourage you to explore our services outlined in this web site.
